카테고리 보관물: JNOPTIC

JNOPTIC

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01_Setup_AcquCAM-3_(2022-08-09)다운로드
05_Setup_AcquCAM 5U3다운로드
27_Setup_AcquCAM ProG3 ＆ ProS3다운로드
29_Setup_AcquCAM 3G다운로드
30_Setup_AcquCAM 5G다운로드
31_Setup_AcquCAM 23GR2&23S2다운로드
31_Setup_AcquCAM 23GR2&23S2다운로드
33_Setup_AcquCAM 23S다운로드
71_JNO-Measure 이미지 분석소프트웨어다운로드
72_(Tdi)Tomoro ScopeEye3.6_배포버젼다운로드
73_Setup_JNO-iXG_for 64bit _(2022-06-02)다운로드
74_JNO-ARM Setup (AquCAM 연동)다운로드
75_Dongle Driver (2021) for JNO-ARM다운로드
83_Setup_AcquCAM II Driver_( 2022-03-07 )다운로드
84_Setup_KON3.1 Driver다운로드

Chroma, Filter, Fluorescence, JNOPTIC

Mycobacterium tuberculosis

결핵 검진을 위한 형광현미경 검경법

Auramine O나 Auramine-rhodamine을 이용한 염색법으로 20x 또는 40x 대물렌즈로 관찰 한다 (접안렌즈 기준 200배 또는 400배). 이러한 형광염색은 Ziehl-Neelsen (ZN) 염색에 비해 민감도가 10% 높아서 관찰량이 많은 사용자에게 권고하는 것이 좋다.

JNO-19008

Ex: 425~475 ㎚, Dm: 485 ㎚, Em: 495 ㎚~
Auramine Longpass

JNOPTIC, Microscope, Physiology

Image Upgrade by JHJIN for Electrophysiology

전기생리학 현미경의 이미지 개선 작업

하단의 이미지는 NIKON의 FN1 현미경의 DIC 세팅을 바르게 맞춘 후의 이미지이다.  상대적으로 OLYMUS의 BX-WI의 Image에 비교하면 미세영역의 분해가 제대로 되지 않은 이미지 임을 알 수 있다.

DIC Image
NIKON FN1 & AcquCAM 23S2

 

이미지 개선 작업 by J.H.JIN

NIKON 현미경 FN1의 장비를 Modify하여  이미지 개선을 실현하였습니다. 상단의 미세 영역의 이미지 분해가 부족한 부분을 상당부분 개선 됨을 하단의 이미지를 통하여 확인 할 수 있습니다.

1차개선 by J.HJIN

DIC Upgrade Image by J.H.JIN
NIKON FN1 & AcquCAM 23S2 & JNO-GGE

2차개선 by J.H.JIN

DIC Upgrade Image by J.H.JIN
NIKON FN1 & AcquCAM 23S2 & JNO-NIR

 

본 작업은 서울대학교 의과대학 생리학교실 중 한 연구실에서 오랜기간 OLYMPUS  BX-WI와 비교하여 매우 떨어진 이미지를 구현해 왔던 니콘의 현미경 FN1을 담당 교수님의 의뢰를 받아 광학개조 작업을 진행 한 결과 이미지입니다.  상기 세 이미지를 보면 원래의 장비에서 이미지에 비하여 상당히 개선된 1차 결과물과 2차 결과물의 이미지를 확인 할 수 있습니다.

아울러 OLYMPUS의 구형현미경인  BX50-WI도 상기 니콘의 현미경인 FN1과 같이 기본 구성으로는 획득 되어지는 이미지의 품질을 기대하기 어렵습니다.  이러한 경우에도 이미지 개선 작업을 통하여 양질의 이미지 획득을 유도 가능합니다.

(적용사례 : 성균관 대학교 의대 생리학 교실 OLYMPUS BX50-WI)

Chroma, Filter, Fluorescence, JNOPTIC

JNO-G(L)

JNOPTIC Filter JNO-G(L)

Download Catalog _ JNO-G(L)

– Filter set for TRITC / Cy3 Longpass
– Chroma Filter Model No. : 19004

JNO-G(L) Spectrum

JNO-G(L)
Standard Green Excitaion filter (Long Pass type)
FiltersType
AT540/25xEX
AT565DCBS
AT575lpEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* TRITC VS JNO-G(L)

JNO-G(L)필터를 이용한 Sample Image 촬영

JNO-G(L)를 사용하여 보는 TRITC 형광 이미지
Filter Set : JNO-G(L)
Camera : AcquCAM 23GR2
C-mount adapter : 1x
Lens : UPLAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
JNO-G(L)를 사용하여 보는 TRITC 형광 이미지
Filter Set : JNO-G(L)
Camera : AcquCAM 5G
C-mount adapter : 1x
Lens : UPLAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
JNO-G(L)를 사용하여 보는 TRITC 형광 이미지
Filter Set : JNO-G(L) Camera : AcquCAM 3G C-mount adapter : 1x Lens : UPLAPO40X Sample : bovine pulmonary artery endothelial cell, BPAEC

JNO-G(L)필터에 적합한 형광색소

  • TRITC
  • Cy3
  • R-phycoerythrin

JNO-G(L) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence, JNOPTIC

JNO-B(L)

JNOPTIC Filter JNO-B(L)

Download Catalog _ JNO-B(L)

– Filter set for GFP / FITC Longpass
– Chroma Filter Model No. : 19002

JNO-B(L) Spectrum

Blue Excitation, Long Pass Emission
JNO-B(L) Spectrum
FiltersType
AT480/30xEX
 AT505DCBS
AT515lpEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

 * FITC VS JNO-B(L)

FITC VS JNO-B(L)

 * GFP VS JNO-B(L)

GFP VS JNO-B(L)
GFP VS JNO-B(L)

JNO-B(L)필터를 이용한 Sample Image 촬영

Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(L) Camera : AcquCAM 23GR2 C-mount adapter : 1X Lens : UPLNAPO40X
AcquCAM 3G BX51 UPlanApo40x JNO-B(L) 1X
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(L) Camera : AcquCAM 3G C-mount adapter : 1X Lens : UPLNAPO40X
Fluorescence Image by AcquCAM 5G
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(L) Camera : AcquCAM 5G C-mount adapter : 1X Lens : UPLNAPO40X

JNO-B(L)필터에 적합한 형광색소

  • Acridine Orange + DNA and RNA
  • Auramine-rhodamine
  • JC-1 non-ratiometric

JNO-B(L) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence, JNOPTIC

JNO-U(L)

Download Catalog _ JNO-U(L)

– Filter set for UV/DAPI Longpass
– Chroma Filter Model No. : 19000

JNO-U(L) Spectrum

JNO-U(L) Spectrum
FiltersType
AT375/28xEX
AT415DCBS
AT435lpEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* DAPI VS JNO-U(L)

DAPI VS JNO-U(L)

JNO-U(L)필터를 이용한 Sample Image 촬영

DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Lens : UPLNAPO40X, C-mount adapter : 1X
Camera : AcquCAM 23GR2, Filter Set : JNO-U(L)
DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Lens : UPLNAPO40X, C-mount adapter : 1X
Camera : AcquCAM 23S2, Filter Set : JNO-U(L)
DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Lens : UPLNAPO40X, C-mount adapter : 1X
Camera : AcquCAM 3G, Filter Set : JNO-U(L)
DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Lens : UPLNAPO40X, C-mount adapter : 1X
Camera : AcquCAM 5G, Filter Set : JNO-U(L)

JNO-U(L)에 적합한 형광색소

  • Calcofluor White
  • DAPI

JNO-U(L) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence, JNOPTIC

JNO-R1(B)

JNOPTIC Filter JNO-R1(B)

Download Catalog_JNO-R1(B)

– Filter set for Texas Red / mCherry / AlexaFluor 594
– Chroma Filter Model No. : 39010

JNO-R1(B) Spectrum

JNO-R1(B)
JNO-R1(B)
FiltersType
AT560/40xEX
AT600DCBS
AT635/60mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

Texas Red VS JNO-R1(B)
Texas Red VS JNO-R1(B)
mCherry VS JNO-R1(B)
mCherry VS JNO-R1(B)
Alexa Fluor 594 VS JNO-R1(B)
Alexa Fluor 594 VS JNO-R1(B)

JNO-R1(B)필터에 적합한 형광색소

JNO-R1(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence, JNOPTIC

JNO-G(B)

JNOPTIC Filter JNO-G(B)

Download Catalog _ JNO-G(B)

– Filter set for TRITC / Cy3 / TagRFP / AlexaFluor546
– Chroma Filter Model No. : 39004

JNO-G(B) Spectrum

JNO-G(B)
Standard Green Excitaion filter (Bans Pass type)
FiltersType
AT540/25xEX
AT565DCBS
AT605/55mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* TRITC VS JNO-G(B)

TRITC VS JNO-G(B) Spetctrum Data
TRITC에 사용했을때 JNO-G(B)

 * Cy3 VS JNO-G(B)

 * AlexaFluor546 VS JNO-G(B)

JNO-G(B) VS AF594
AF594에 사용되는 JNO-G(B)의 스펙트럼 비교

JNO-G(B)필터를 이용한 Sample Image 촬영

JNO-G(B)를 사용하여 보는 TRITC 형광 이미지
Filter Set : JNO-G(B)
Camera : AcquCAM 3G
C-mount adapter : 1x
Lens : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC
대체 텍스트
Filter Set : JNO-G(B)
Camera : AcquCAM 5G
C-mount adapter : 1x
Lens : UPLAPO40X
Sample : bovine pulmonary artery endothelial cell, BPAEC
JNO-G(B)를 사용하여 보는 TRITC 형광 이미지
Filter Set : JNO-G(B)
Camera : AcquCAM 23GR2
C-mount adapter : 1x
Lens : UPLAPO20X
Sample : bovine pulmonary artery endothelial cell, BPAEC

JNO-G(B)필터에 적합한 형광색소

JNO-G(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Chroma, Filter, Fluorescence, JNOPTIC

JNO-B(B)

JNOPTIC Filter JNO-B(B)

Download Catalog _ JNO-B(B)

– Filter set for EGFP / FITC / Cy2 / AlexaFluor488
– Chroma Filter Model No. : 39002

JNO-B(B) Spectrum

FiltersType
AT480/30xEX
 AT505DCBS
AT535/40mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* FITC VS JNO-B(B)

FITC VS JNO-B(B)

 * GFP VS JNO-B(B)

JNO-B(B) VS GFP spectrum 비교
JNO-B(B) VS GFP spectrum chart

 * AlexaFluor488 VS JNO-B(B)

JNO-B(B) VS AlexaFluor 488
JNO-B(B) VS AlexaFluor 488

JNO-B(B)필터를 이용한 Sample Image 촬영

Fluorescence Image by AcquCAM 23GR2
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(B) Camera : AcquCAM 23GR2 C-mount adapter : 1X Lens : PlanApo60xWLSM
Fluorescence Image by AcquCAM 3G
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(B) Camera : AcquCAM 3G C-mount adapter : 1X Lens : PlanApo60xWLSM
Fluorescence Image by AcquCAM 5G
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(B) Camera : AcquCAM 5G C-mount adapter : 1X Lens : UPLNAPO40X

JNO-B(B)필터에 적합한 형광색소

JNO-B(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing. ( Tel. 02-3473-4188)