카테고리 보관물: Fluorescence

Chroma, Filter, Fluorescence

49009

Chroma Filter 49009

49009 Spectrum

Chroma_49009
Chroma_49009
FiltersType
ET640/30xEX
 T660lpxrBS
ET690/50mEM

– Filter set for Cy5 Narrow Excitation

The narrow-band excitation provided by this filter set is useful in applications where fluorochromes such as Texas Red or AlexaFluor 594 are also present in the same sample. This set will minimize bleedthrough of these fluorochromes. Best FISH filter set for Far Red probes.

  • Main Fluorochromes :Cy5
Excitation (㎚)Emission (㎚)
649670

Spectrum Comparison Fluor and 49009

Cy5 VS 49009
Cy5 VS 49009

Best with these fluorochromes

49009 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Filter, Fluorescence

49008

JNOPTIC Filter 49008

49008 Spectrum

Chroma_49008
Chroma_49008
FiltersType
ET560/40xEX
 T585lpxrBS
ET630/75mEM

– Filter set for Alexa Fluor 594, mCherry, Texas Red

Excitation (㎚)Emission (㎚)
590617

Spectrum Comparison Fluor and 49008

Alexa Fluor 594 VS 49008
Alexa Fluor 594 VS 49008

Best with these fluorochromes

49008 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Filter, Fluorescence

49007

Chroma Filter 49007

49007 Spectrum

Chroma_49007
Chroma_49007
FiltersType
ET710/75xEX
 T760lpxrBS
ET810/90mEM

– Filter set for Cy7

  • Main Fluorochromes : Cy7™
Excitation (㎚)Emission (㎚)
743767

Spectrum Comparison Fluor and 49007

Cy7 VS 49007
Cy7 VS 49007

Best with these fluorochromes

49007 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

Chroma, Filter, Fluorescence

49002

Chroma Filter 49002

49002 Spectrum

Chroma_49002
Chroma_49002
FiltersType
ET470/40xEX
 T495lpxrBS
ET525/50mEM

– Filter set for EGFP (FITC/Cy2)

  • Main Fluorochromes : GFP
Excitation (㎚)Emission (㎚)
488507

Spectrum Comparison Fluor and 49002

GFP VS 49002
GFP VS 49002

Best with these fluorochromes

49002 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm or 3.5mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm or 25.5×36, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Filter, Fluorescence

39009

Chroma Filter 39009

39009 Spectrum

Chroma_39009
Chroma_39009
FiltersType
AT480/30xEX
 AT505DCBS
AT620/60mEM

– Filter set for Acridine Orange/Di-8-ANEPPS

  • Main Fluorochromes : EtBr
Excitation (㎚)Emission (㎚)
520603

Spectrum Comparison Fluor and 39009

EtBr VS 39009
EtBr VS 39009

Best with these fluorochromes

  • Acridine Orange + RNA
  • Di-8-ANEPPS non-ratiometric
  • DiA
  • Ethidium Bromide
  • FM™ 1-43
  • LysoTracker Yellow HCK-123
  • Pulsar™ 650

39009 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm or 3.5mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm or 25.5×36, 1mm thick, to fit standard microscope manufacturer filter cubes

Chroma, Filter, Fluorescence

39003

Chroma Filter 39003

39003 Spectrum

Chroma_39003
Chroma_39003
FiltersType
AT495/20xEX
 AT515DCBS
AT540/30mEM

– Filter set for EYFP/Venus/Citrine

  • Main Fluorochromes : EYFP
Excitation (㎚)Emission (㎚)
514527

Spectrum Comparison Fluor and 39003

EYFP VS 39003
EYFP VS 39003

Best with these fluorochromes

39003 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm or 3.5mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm or 25.5×36, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Filter, Fluorescence

39001

JNOPTIC Filter 39001

Download Catalog_39001

39001 Spectrum

FiltersType
AT435/20xEX
 AT455DCBS
AT480/30mEM

– Filter set for ECFP/Cerulean

  • Main Fluorochromes : CFP
Excitation (㎚)Emission (㎚)
433475

Spectrum Comparison Fluor and 39001

CFP VS 39001
CFP VS 39001

Best with these fluorochromes

  • AmCyan
  • Atto 425
  • Cerulean
  • CFP
  • ECFP

39001 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Fluorescence, Fluorochrome

Cy5.5

Spectrum Chart

Cy5.5 Spectrum

Excitation (㎚) Emission (㎚)
675 695

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49022 545/25
(532.5~557.5)		 565 (LP) 605/70
(570~640)		 Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49914 642/20
(632~652)		 647 (LP) 705/72
(669~741)		 For use with laser between 633~650㎚

Narrow Excitation band,
Bandpass Emission

Information

A variety of cyanine 5.5 (Cy5.5) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5 dyes are one type of the most common red fluorophores.

Cyanine 5.5 (Cy5.5) 염료는 형광 이미징 및 기타 형광 기반 생화학 분석을 위해 생물 분자를 표지하는 데 사용되었습니다. 그들은 펩타이드, 단백질 및 올리고 제 등의 라벨링에 널리 사용됩니다. Cy5.5 염료는 가장 일반적인 적색 형광체의 한 유형입니다.

Fluorescence, Fluorochrome

tdTomato

Spectrum Chart

Excitation (㎚) Emission (㎚)
554 581

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)		 565 (LP) 605/55
(577.5~632.5)		 Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)		 565 (LP) 605/70
570~640)		 Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)		 565 (LP) 590/33
(573.5~606.5)		 Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49908 532/10
(527~537)		 532 (LP) 590/50
(565~615)		 For use with laser between 529~535㎚

Narrow Excitation band,
Longpass Emission
49909 561/10
(556~566)		 561 (LP) 600/50
(575~625)		 For use with laser between 557~563㎚

Narrow Excitation band,
Bandpass Emission

Information

tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation. Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato. Its excitation and emission maxima occur at 554 ㎚ and 581 ㎚, respectively. Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.

Fluorescence, Fluorochrome

EYFP

Spectrum Chart

EYFP Spectrum
EYFP Spectrum
EX(㎚)EM(㎚)
514527

Recommended Filter set

  • 일반 형광 Lighting source
Filter Model No.Excitation filter (㎚)Dichroic mirror (㎚)Emission filter (㎚)Remarks
39003495/20
(485~505)		515 (LP)535/30
(520~550)		Narrow Excitation band,
Bandpass Emission
49003500/20
(490~510)		515 (LP)540/30
(525~555)		Narrow Excitation band,
Bandpass Emission
  • 레이저 Lighting source 
Filter Model No.Excitation filter (㎚)Dichroic mirror (㎚)Emission filter (㎚)Remarks
49905514/10
(509~519)		514 (LP)545/40
(525~565)		For use with laser between 511~517㎚ Narrow Excitation band,
Bandpass Emission

Information

Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 ㎚ and its emission peak is 527 ㎚. Like the parent GFP, YFP is a useful tool in cell and molecular biology thanks to its properties useful for fluorescence microscopy.

노란색 형광 단백질 (YFP)은 원래 해파리 Aequorea victoria에서 파생 된 녹색 형광 단백질 (GFP)의 유전 적 돌연변이입니다. 그것의 여기 피크는 514 ㎚이고 방출 피크는 527 ㎚ 입니다. 부모 GFP와 마찬가지로 YFP는 형광 현미경 검사에 유용한 그 특성 덕분에 세포 및 분자 생물학에서 유용한 도구입니다.