mCherry

Spectrum Chart

mCherry Spectrum
mCherry Spectrum
Excitation (㎚)Emission (㎚)
587610

Recommended Filter set

  • 일반 형광 Lighting source 사용시
Filter Model No.Excitation filter (㎚)Dichroic mirror (㎚)Emission filter (㎚)Remarks
JNO-R1(B)560/40
(540~580)
600 (LP)635/60
(605~665)
Wide Excitation band,
Bandpass Emission
49008560/40
(540~580)
600 (LP)630/75
(592.5~667.5)
Wide Excitation band,
Bandpass Emission
49306580/25
(567.5~592.5)
600 (LP)625/30
(610~640)
Narrow Excitation band,
Bandpass Emission
  • 레이저 Lighting source 사용시
Filter Model No.Excitation filter (㎚)Dichroic mirror (㎚)Emission filter (㎚)Remarks
49910561/10
(556~565)
561 (LP)630/70
(595~665)
For use with laser between 557~563 Narrow Excitation band,
Bandpass Emission
49911594/10
(589~599)
594 (LP)645/75
(607.5~682.5)
For use with laser between 591~596 Narrow Excitation band,
Bandpass Emission

Information

mCherry is a fluorophore (a fluorescent protein) used as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from the protein drFP583 (commonly known as DsRed) isolated from Discosoma sea anemones, while other fluorescent proteins in the green range are often variants of jellyfish GFP. DsRed is a tetramer optimally excited at 558 ㎚ and has an emission maximum at 583 ㎚.

mCherry는 형광 물질 (형광 단백질)로서 분자 및 세포 구성 요소에 태그를 부착 할 때 marker 로 사용됩니다. mCherry와 대다수의 적색 형광 단백질은 Discosoma 말미잘에서 분리된 단백질 drFP583 (일반적으로 DsRed라고 함)에서 유래한 반면, 녹색 범위의 다른 형광 단백질은 종종 해파리 GFP의 변종입니다. DsRed는 558 ㎚에서 최적으로 여기되고, 583 ㎚ 에서 최대 방출을 갖는 4 량체입니다.