카테고리 보관물: Fluorochrome

Chroma, Filter, Fluorescence, Fluorochrome

FISH

Fluorescence In Situ Hybridization
– 형광동소보합법 –

Inadequat Contrast Image for FISH
Inadequat Contrast Image
Good Contrast Image for FISH
Good Contrast Image
Inadequat Contrast Spectrum for FISH Filter
Inadequat Contrast Spectrum Chart
Good Contrast Spectrum Chart for FISH Filter
Good Contrast Spectrum Chart

Many factors influence the quality of FISH

1. Processing & storage2. Specimen3. Probes
4. Microscope5. Filters6. Light source
Many factors influence the quality of FISH
Inadequat Contrast Image for FISHGood Contrast Image for FISH
필터 선정시 고려 할 사항설 명
ContrastSignal / Noise ratio =
MORE CONTRAST
BrightnessSave time, increase throughput
and reduce eye strain with brighter images
Color SeparationDistinguish between colors
more easily and exclude unwanted colors
RegistrationEach filter set produces images that are aligned with images from other filter sets
형광 필터 선정시 고려할 포인트

Chroma, Fluorescence, Fluorochrome

69302

Chroma Filter 69302

ET-DAPI/FITC/Texas Red® with single band exciters

69302 Spectrum

Chroma_69302
Chroma_69302
FiltersType
ET402/15xEX
ET490/20xEX
ET572/35xEX
69002bsBS
69002mEM

69302 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.

Chroma, Fluorescence, Fluorochrome

69015

Chroma Filter 69015

ET-DAPI/Green/Red FISH

Triple band filter set ideal for simultaneous viewing of DAPI and commercially available Green and Red FISH probes. Attenuated level of DAPI excitaiton provides better balance of colors so that DAPI signal is not overwhelming.

69015 Spectrum

Chroma_69015
Chroma_69015
FiltersType
69015xEX
69015bsBS
69015mEM

69015 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.

Fluorescence, Fluorochrome

X-rhod-1/Ca2+

Spectrum Chart

X-rhod-1/Ca2+ Spectrum

Excitation (㎚) Emission (㎚)
580 601

 

Recommended Filter Set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-R1(B) 560/40
(540~580)		 600 (LP) 635/60
(605~665)		 Wide Excitation band,
Bandpass Emission
49008 560/40
(540~580)		 600 (LP) 630/75
(592.5~667.5)		 Wide Excitation band,
Bandpass Emission
49306 580/25
(567.5~592.5)		 600 (LP) 625/30
(610~640)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source -> 문의 주시기 바랍니다.

 

Fluorescence, Fluorochrome

TurboFP650

Spectrum Chart

TurboFP650 Spectrum

Excitation (㎚) Emission (㎚)
592 650

 

Recommended Filter Set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
19006 560/40
(540~580)		 600 (LP) 610 (LP) Wide Excitation band,
Bandpass Emission
49008 560/40
(540~580)		 600 (LP) 630/75
(592.5~667.5)		 Wide Excitation band,
Bandpass Emission
49017 560/40
(540~580)		 590 (LP) 590 (LP) Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49911 594/10
(589~599)		 594 (LP) 645/75
(607.5~682.5)		 For use with laser between 591~596㎚

Narrow Excitation band,
Bandpass Emission
49912 594/10
(589~599)		 594 (LP) 610 (LP) For use with laser  594㎚

Narrow Excitation band,
Longpass Emission

 

 

Fluorescence, Fluorochrome

TO-PRO-3

Spectrum Chart

TO-PRO-3 Spectrum

Excitation (㎚) Emission (㎚)
642 661

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-R2(B) 620/50
(595~645)		 655 (LP) 690/50
(665~715)		 Wide Excitation band,
Bandpass Emission
49006 620/60
(590~650)		 660 (LP) 700/75
(662.5~737.5)		 Very Wide Excitation band,
Bandpass Emission
49009 640/30
(625~655)		 660 (LP) 690/50
(665~715)		 Medium Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시 -> 문의 주시기 바랍니다.

 

Fluorescence, Fluorochrome

Topaz

Spectrum Chart

Topaz Spectrum

Excitation (㎚) Emission (㎚)
514 529

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
39003 495/20
(485~505)		 515 (LP) 535/30
(520~550)		 Narrow Excitation band,
Bandpass Emission
49003 500/20
(490~510)		 515 (LP) 540/30
(525~555)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source  사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49904 488/10
(483~493)		 488 (LP) 525/50
(500~550)		 For use with laser between 485~491㎚

Very Narrow Excitation band,
Bandpass Emission
49905 514/10
(509~519)		 514 (LP) 545/40
(525~565)		 For use with laser between 511~517㎚

Narrow Excitation band,
Bandpass Emission

 

Fluorescence, Fluorochrome

Tetracycline

Spectrum Chart

 

 

Excitation (㎚) Emission (㎚)
405 550

 

Recommended Filter Set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49026 405/40
(385~425)		 470 (LP) 550/60
(520~580)		 Wide Excitation band,
Longpass Emission

 

  • 레이저 Lighting source -> 문의 주시기 바랍니다.

 

 

 

Fluorescence, Fluorochrome

TET

Spectrum Chart

TET Spectrum

Excitation (㎚) Emission (㎚)
521 536

 

Recommended Filter Set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49023 480/40
(460~500)		 510 (LP) 535/50
(510~560)		 Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source -> 문의 주시기 바랍니다.

 

 

 

Fluorescence, Fluorochrome

TAMRA

Spectrum Chart

TAMRA Spectrum

Excitation (㎚) Emission (㎚)
555 580

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)		 565 (LP) 605/55
(577.5~632.5)		 Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)		 565 (LP) 605/70
570~640)		 Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)		 565 (LP) 590/33
(573.5~606.5)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49909 561/10
(556~566)		 561 (LP) 600/50
(575~625)		 For use with laser between 557~563㎚

Narrow Excitation band,
Bandpass Emission