Spectrum Chart
| Excitation (㎚) | Emission (㎚) |
| 490 | 525 |
Recommended Filter
-
일반 형광 Lighting source 사용시
| Filter Model No. | Excitation filter (㎚) | Dichroic mirror (㎚) | Emission filter (㎚) | Remarks |
| JNO-B(B) | 480/30 (465~495) |
505 (LP) | 535/40 (515~555) |
Medium Excitation band, Bandpass Emission |
| 49002 | 470/40 (450~490) |
495 (LP) | 525/50 (500~550) |
Wide Excitation band, Bandpass Emission |
| 49011 | 480/40 (460~500) |
510 (LP) | 535/50 (510~560) |
Wide Excitation band, Bandpass Emission |
-
레이저 Lighting source 사용시
| Filter Model No. | Excitation filter (㎚) | Dichroic mirror (㎚) | Emission filter (㎚) | Remarks |
| 49904 | 488/10 (483~493) |
488 (LP) | 525/50 (500~550) |
For use with laser between 485~491㎚
Very Narrow Excitation band, |
Information
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluoresce in molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
A succinimidyl-ester functional group attached to the fluorescein core, creating “NHS-fluorescein”, forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles.
FITC has excitation and emission spectrum peak wavelengths of approximately 495 ㎚ /519 ㎚. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.