Fliter Table for CoolLED illuminations.
| Single Band Sets | pE-300 Series | pE-4000 | pE-340fura | |
|---|---|---|---|---|
| DAPI | 39000 SB (UV@365) | 385 (Ch.A) | 49028 | 49028 |
| DAPI | 49028 MB (UV@400) | |||
| CFP | 49013 | 435 (Ch.A) | 49001 | 49013 |
| GFP/FITCS | 49002 | 460 (Ch.B) | 49002 | 49002 |
| YFP | 49023 | 500 (Ch.C) | 49003 | 49023 |
| TRITC/Cy3 | 49004 | 550 (Ch.C) | 49004 | 49004 |
| TxRed/mCherry | 49008 | 550 (Ch.C) | 49008 | 49008 |
| Cy5 | 49006 | 635 (Ch.D) | 49009 | 49006 |
| Cy7/IrDye800 | N/A | 740 (Ch.D) | 49007 | N/A |
| Full Multi Band Sets | pE-300 Series | pE-4000 | pE-340fura | |
|---|---|---|---|---|
| DAPI/FITC/TRITC | 69013v2 | 385/470/550 (Ch.A.B.C) |
69013v2 | 69013v2**** |
| DAPI/FITC/TxRED | 69013v2 | 385/490/580 (Ch.A.B.C) |
69015** | 69013v2**** |
| DAPI/FITC/TRITC/Cy5 | 89402* | 385/460/550/635 (Ch.A.B.C.D) |
89402 | 89402**** |
| GFP/mCherry | 59022 | 460/550(Ch.B.C) | 59022 | 59022***** |
| CFP/YFP | 59017*** | 435/500(Ch.A.B) | 59017 | 59017*** |
| CFP/YFP/mCherry | 69008*** | 435/500/580 (Ch.A.B.C) |
69008 | 69008***** |
| CFP/YFP/RFP/Cy7 | N/A | N/A | 89403 | N/A |
| Pinkel Sets | pE-300ultra MB(UV@400) | pE-4000 | pE-340fura | |
|---|---|---|---|---|
| Chroma | LED | Chroma | Chroma | |
| FURA-2 | N/A | N/A | N/A | 79001 |
| FURA-2/GFP | N/A | N/A | N/A | 79004 |
| FURA-2/TRITC | N/A | N/A | N/A | 79003 |
| DAPI/FITC/TRITC | 69302 | 405/470/580 (Ch.A.B.C) |
69302 | N/A |
| DAPI/FITC/TxRED | 69302 | 405/470/580 (Ch.A.B.C) |
69302 | N/A |
| DAPI/FITC/TRITC/Cy5 | N/A | 405/470/550/635 (Ch.A.B.C.D) |
89400 | N/A |
| GFP/mCherry | 59222 | 460/580/ (Ch.B.C) |
59222 | N/A |
| CFP/YFP | 59217*** | 435/500 (Ch.A.B) |
59217 | N/A |
| CFP/YFP/mCherry | 69308*** | 435/500/580 (Ch.A.B.C) |
69308 | N/A |
| * | pE-300lite will allow vis. Of all 4. pE-300white & pE-300ultra will allow manual and software control of DAvsFIvsTR&Cy5 |
| ** | Very specific to TxRed/mCherry/Red FISH and but not recomended for TRITC/Cy3 etc.. |
| *** | Only recommended for stronger YFP signals |
| **** | All visable, only independent control of DAPI – others via single source in the pE-340fura |
| ***** | All visiable but via same source -so no switching or balancing |