카테고리 보관물: Fluorochrome

Fluorescence, Fluorochrome

AlexaFluor 555

Spectrum Chart

AlexaFluor 555 Spectrum
Excitation (㎚) Emission (㎚)
553 568

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)		 565 (LP) 605/55
(577.5~632.5)		 Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)		 565 (LP) 605/70
570~640)		 Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)		 565 (LP) 590/33
(573.5~606.5)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49907 532/10
(527~537)		 532 (LP) 575/50
(550~600)		 For use with laser between 529~535㎚

Narrow Excitation band,
Bandpass Emission
49908 532/10
(527~537)		 532 (LP) 590/50
(565~615)		 For use with laser between 529~535㎚

Narrow Excitation band,
Bandpass Emission

 

 

 

 

 

 

 

 

 

Fluorescence, Fluorochrome

AlexaFluor 546

Spectrum Chart

AlexaFluor 546 Spectrum
Excitation (㎚) Emission (㎚)
556 573

Recommended Filter

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)		 565 (LP) 605/55
(577.5~632.5)		 Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)		 565 (LP) 605/70
570~640)		 Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)		 565 (LP) 590/33
(573.5~606.5)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49908 532/10
(527~537)		 532 (LP) 590/50
(565~615)		 For use with laser between 529~535㎚

Narrow Excitation band,
Longpass Emission
49909 561/10
(556~566)		 561 (LP) 600/50
(575~625)		 For use with laser between 557~563㎚

Narrow Excitation band,
Bandpass Emission

 

 

Fluorescence, Fluorochrome

AlexaFluor 532

Spectrum Chart

AlexaFluor 532 Spectrum
Excitation (㎚) Emission (㎚)
531 554

 

Recommended Filter set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49014 530/30
(515~545)		 550 (LP) 575/40
(555~595)		 Medium Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49906 514/10
(509~519)		 514 (LP) 570/50
(545~595)		 For use with laser between 511~517㎚

Narrow Excitation band,
Bandpass Emission
49907 532/10
(527~537)		 532 (LP) 575/50
(550~600)		 For use with laser between 529~535㎚

Narrow Excitation band,
Bandpass Emission

 

 

 

Fluorescence, Fluorochrome

AlexaFluor 514

Spectrum Chart

AlexaFluor 514 Spectrum

Excitation (㎚) Emission (㎚)
517 539

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
39003 495/20
(485~505)		 515 (LP) 535/30
(520~550)		 Narrow Excitation band,
Bandpass Emission
49003 500/20
(490~510)		 515 (LP) 540/30
(525~555)		 Narrow Excitation band,
Bandpass Emission
49023 480/40
(460~500)		 510 (LP) 535/50
(510~560)		 Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source  사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49905 514/10
(509~519)		 514 (LP) 545/40
(525~565)		 For use with laser between 511~517㎚

Narrow Excitation band,
Bandpass Emission
49906 514/10
(509~519)		 514 (LP) 570/50
(545~595)		 For use with laser between 511~517㎚

Narrow Excitation band,
Bandpass Emission

 

 

 

Fluorescence, Fluorochrome

AlexaFluor 488

Spectrum Chart

AlexaFluor 488 Spectrum
Excitation (㎚) Emission (㎚)
498 520

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-B(B) 480/30
(465~495)		 505 (LP) 535/40
(515~555)		 Medium Excitation band,
Bandpass Emission
49002 470/40
(450~490)		 495 (LP) 525/50
(500~550)		 Wide Excitation band,
Bandpass Emission
49011 480/40
(460~500)		 510 (LP) 535/50
(510~560)		 Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49904 488/10
(483~493)		 488 (LP) 525/50
(500~550)		 For use with laser between 485~491㎚

Narrow Excitation band,
Bandpass Emission

 

 

Fluorescence, Fluorochrome

AlexaFluor 350

Spectrum Chart

AlexaFluor 350 Spectrum
Excitation (㎚) Emission (㎚)
346 442

 

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-U(B) 375/28
(361-389)		 415 (LP) 460/50
(435-485)		 Narrow Excitation band,
Bandpass Emission
49000 350/50
(325-375)		 400 (LP) 460/50
(435-485)		 Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source  사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49915 365/20
(355~375)		 365 (LP) 425 (LP) Narrow Excitation band,
longpass Emission

 

 

Fluorescence, Fluorochrome

Acridine Orange+DNA

Spectrum Chart

Acridine Orange+DNA Spectrum
Excitation (㎚) Emission (㎚)
500 526

 

Recommended Filter Set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-B(B) 480/30
(465~495)		 505 (LP) 535/40
(515~555)		 Medium Excitation band,
Bandpass Emission
49002 470/40
(450~490)		 495 (LP) 525/50
(500~550)		 Wide Excitation band,
Bandpass Emission
49011 480/40
(460~500)		 510 (LP) 535/50
(510~560)		 Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source -> 문의 주시기 바랍니다.

 

 

Fluorescence, Fluorochrome

mCherry

Spectrum Chart

mCherry Spectrum
Excitation (㎚) Emission (㎚)
587 610

 

Recommended Filter set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-R1(B) 560/40
(540~580)		 600 (LP) 635/60
(605~665)		 Wide Excitation band,
Bandpass Emission
49008 560/40
(540~580)		 600 (LP) 630/75
(592.5~667.5)		 Wide Excitation band,
Bandpass Emission
49306 580/25
(567.5~592.5)		 600 (LP) 625/30
(610~640)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49910 561/10
(556~565)		 561 (LP) 630/70
(595~665)		 For use with laser between 557~563㎚

Narrow Excitation band,
Bandpass Emission
49911 594/10
(589~599)		 594 (LP) 645/75
(607.5~682.5)		 For use with laser between 591~596㎚

Narrow Excitation band,
Bandpass Emission

Information

mCherry is a fluorophore (a fluorescent protein) used as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from the protein drFP583 (commonly known as DsRed) isolated from Discosoma sea anemones, while other fluorescent proteins in the green range are often variants of jellyfish GFP. DsRed is a tetramer optimally excited at 558 ㎚ and has an emission maximum at 583 ㎚.

mCherry는 형광 물질 (형광 단백질)로서 분자 및 세포 구성 요소에 태그를 부착 할 때 marker 로 사용됩니다. mCherry와 대다수의 적색 형광 단백질은 Discosoma 말미잘에서 분리된 단백질 drFP583 (일반적으로 DsRed라고 함)에서 유래한 반면, 녹색 범위의 다른 형광 단백질은 종종 해파리 GFP의 변종입니다. DsRed는 558 ㎚에서 최적으로 여기되고, 583 ㎚ 에서 최대 방출을 갖는 4 량체입니다. 
Fluorescence, Fluorochrome

EBFP

Spectrum Chart
BFP Spectrum
Excitation (㎚) Emission (㎚)
385 448
Recommended Filter set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49021 405/20
(395~415)		 425 (LP) 460/50
(435~485)		 Narrow Excitation band,
Bandpass Emission
49028 395/25
(382.5~407.5)		 425 (LP) 460/50
(435~485)		 Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49901 405/20
(395~415)		 405 (LP) 460/50
(435~485)		 For use with laser between 400~410㎚

Narrow Excitation band,
Bandpass Emission

Information

Fluorescent proteins, like EBFP, can be used as protein “tags” to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems.

EBFP와 같은 형광 단백질은 자극이나 전이를 위한 안정적인 marker 로서 또는 그들의 일시적인 발현의 하위 세포를 연구하기 위해 단백질 “태그” 로 사용될 수 있습니다.