결핵 검진을 위한 형광현미경 검경법
Auramine O나 Auramine-rhodamine을 이용한 염색법으로 20x 또는 40x 대물렌즈로 관찰 한다 (접안렌즈 기준 200배 또는 400배). 이러한 형광염색은 Ziehl-Neelsen (ZN) 염색에 비해 민감도가 10% 높아서 관찰량이 많은 사용자에게 권고하는 것이 좋다.
Auramine O나 Auramine-rhodamine을 이용한 염색법으로 20x 또는 40x 대물렌즈로 관찰 한다 (접안렌즈 기준 200배 또는 400배). 이러한 형광염색은 Ziehl-Neelsen (ZN) 염색에 비해 민감도가 10% 높아서 관찰량이 많은 사용자에게 권고하는 것이 좋다.
270 ㎚ 투과필터 ( 263 ~ 277 ㎚ ) | 350 ㎚ 투과필터( 325 ~ 375 ㎚ ) |
780 ㎚ 이상 투과필터 ( 780 ㎚ ~ ) | 밴드투과 필터 ( 771 ~ 851 ㎚ ) |
AcquCAM 23GR2 칼라 카메라 – 근적외선 대응 – | Color Camera UV 자극을 이용한 형광 이미지 _ DAPI |
AcquCAM 23S2 흑백 카메라 – 근적외선 대응 – | Monochrome Camera ( IR-DIC Imagea ) 적외선을 이용한 미분 간섭 관찰 이미지 |
ET – Fura 2/GFP
Filters | Type |
ET340x | EX |
ET380x | EX |
ET470/40x | EX |
T495lpxru | BS |
ET535/70m | EM |
79004 Spec
NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.
ET – Fura 2/TRITC
Filters | Type |
ET340x | EX |
ET380x | EX |
ET555/25x | EX |
79003bs | BS |
79003m | EM |
79003 Spec
NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.
ET – Fura 2
Filters | Type |
ET340x | EX |
ET380x | EX |
T400lp | BS |
ET510/80m | EM |
79001 Spec
NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.
ET – 436-28/506-21/578-24/730-40 Multi LED set
Quad band catalog set for Multi LED light engines like those manufactured by Lumencor, CoolLED, Excelitas and Cairn Research. Excitation passbands are matched to discrete, triggerable LEDs appropriate for excitation of CFP/YFP/RFP/Cy7 and similar fluors. Not intended for use with white light sources.
Filters | Type |
89403x | EX |
89403bs | BS |
89403m | EM |
89403 Spec
NOTE: These require the use of filter wheels or sliders, or image-splitting devices to hold multiple individual filters.
ET – ECFP/EYFP with single band exciters
Filters | Type |
ET430/24x | EX |
ET500/20x | EX |
69008bs | BS |
59017m | EM |
59217 Spec
NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.
ET – EGFP/mCherry (or FITC/TxRed) with single band exciters
Filters | Type |
ET470/40x | EX |
ET572/35x | EX |
59022bs | BS |
59022m | EM |
59222 Spec
NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.
ET – ECFP/EYFP
Filters | Type |
59017x | EX |
69008bs | BS |
59017m | EM |
59017 Spec
NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.
ET – EGFP/mCherry (or FITC/TxRed)
Filters | Type |
59022x | EX |
59022bs | BS |
59022m | EM |
59022 Spec
NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera.