cell^TIRF

 

Cell^TIRF 소개

동시획득을 위한 OLYMPUS cell^TIRF Motorized Multicolor TIRFs

Objective-based TIRF microscopy 의 선도주자인 OLYMPUS는 multicolor TIRF 분야에서 중요한 도약을 발표하였습니다. 올림푸스 cell^TIRF 광원은 동시에 일어나는 이미지캡쳐를 위한 4개의 전동 채널들을 제공합니다. TIRF 매개변수들의 직관적인 소프트웨어 제어는 widefiled 형광과 TIRF 각도 및 끊김없는 이동의 즉각적인 설정과 확정을 가능하게 합니다. Cell^TIRF 광원으로 각 레이저파장은 최적으로 포커싱되고 각각의 각도는 개별적으로 설정, 다른 파장들이 같은 penetration depth를 가질수 있게 합니다. 사용자는 한번의 마우스 클릭으로 모든 레이져들의 계산된 penetration depths들을 미리 설정할 수 있습니다.
시스템은 개별적으로 모든 채널들로부터 TIRF 정보를 동시에 캡쳐하도록 각 레이저의 각도를 조절합니다.

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특징 및 이득

Features and Benefits

● 유일한 진정한 동시 획득의 4 라인 시스템

● TIRF 각도의 전동 제어

● 정밀한 각도 조절

●  Penetration depth의 소프트웨어 계산

●  소프트웨어 기반의 레이저 감쇠 기능

●  첫번째 레이저 라인의 통합된 Point FRAP 광학계

●  끊김없는 widefield/TIRF 스위칭

●  NA 1.65 의 세계 최고의 6개의 전용 TIRF 렌즈

●  405 ~ 640nm 레이저 시스템

– 최적의 파장을 선택할 수 있는 유연성

●  340nm 투과의 TIRF 와 widefield 혹은 100% widefield

●  각 레이저 라인에 대한 25mm 필터 이용

●  각 레이저 라인에 대한 조절 가능한 Filed stop

●  단단한 단일 알루미늄 프레임

OLYMPUS cell^TIRF Publications

OLYMPUS cell^TIRF Gallery

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Specifications

TIRF illuminator	Available laser Systems	405nm, 445nm, 473nm, 488nm, 515nm, 532nm, 561nm, 594nm, 640nm
	Laser coupling	FC or FC8 connector
	Field Number	FN11.8
	Illuminator Body	Single Billet Aluminum
	Safety	Integrated Interlock
Laser systems	Wavelengths	405nm, 445nm, 473nm, 488nm, 515nm, 532nm, 561nm, 594nm, 640nm
	Power	Laser system available up to 100mW
	Attenuation	Software control of laser intensity
	Imaging Shutter	High speed 1msec TTL shutters
	Safety	Integrated interlock
Epi-Fluorescence	Light source	100W Mercury, 75W Xenon, Metal halide.
	Selection	TIRF/Brightfield or Brightfield only with 340nm transmission
	Field number	FN22
TIRF Objectives	60X Objectives
	PLANAPON60XOTIRFM	PLANAPO 60X, NA 1.45, WD 0.1mm, Correction collar
	APON60XOTIRFM	APO 60X, NA 1.49, WD 0.1mm, Correction collar
	100X Objective
	PLAPO100XOTIRFM	PLANAPO 100X, NA 1.45 WD 0.10mm
	UAPON100XTIRF	UAPON 100X, NA 1.49 WD 0.09mm, Correction collar
	AP0100XO-HR-SP	APO 100X, NA 1.65, WD 0.10mm
	150X Objectives
	UAPON150XOTIRF	UAPO 150X, NA 1.45,WD 0.07mm,Correction collar
Immersion Oil	Type-F	Low Auto Fluorescence Immersion oil
Coverslips	High Refractive Index	RI 1.78 Immersion oil for use with AP0100XO-HR-SP 1.65NA 100X objective
Compatible microscopes

Inclusion Inspector

 

비금속 개재물 표준 검사 시스템  “Inclusion Inspector”

철 내의 비금속 개재물은 깨지기쉬움(brittleness) 및 광범위한 크랙(crack) 형성과 같은 위험하고 심각한 재질 결점을 유발 할 수 있습니다. 비금속 개재물은 sulfidic 혹은 oxidic 성분으로 용해(melting) 공정에서 발생됩니다. 모든 철강들은 많거나 적은 양의 비금속 개재물을 포함합니다.

이러한 비금속 개재물들의 타입 및 출현은 철의 타입, 용해 공정 및 완성 제품의 잉곳(ingot) 혹은 주조라인 (casting strand)의 형상과 같은 요소에 좌우됩니다.

이것은 철이 얼마나 순수한지를 판단하는 특별한 의미입니다.

이것은 sulfidic 및 oxidic 비금속개재물 존재량에 대한 정보를 제공합니다.

Features and Benefits:

• Complete classification of inclusions
• Reliable detection of alumina and silicate types
• Simultaneous anaysis of sulfides and oxides
• JK rating of sulfides, alumina and silicates
• Standards : ISO 4967, ASTM E45, DIN 50602,  JIS G0555, EN 10247, UNI 3244, NF A04-106 and GB10561
• Inclusion size calculation independent of field of view

Filter Inspector

 

올림푸스 자동 필터 분석 시스템 “Filter Inspector”은 필터상의 잔여물을 파악하고 분류하는 미국 및 국가 표준들의 다양한 요구에 부합하기 위해 개발되었습니다.

이 시스템은 2분 이내에 자동적으로 필터(일반적으로 47mm 직경)를 스캔하고 분석 합니다.  잔여물의 광학 이미지가 생성되고 ISO및 USP 표준에 부합하는 보고서 형식에 카운트/분류/문서화 됩니다.

이 과정은 빠르고 오퍼레이터의 실수를 최소화합니다.

필터 분석은 다음과 같은 어플리케이션에서 사용됩니다.
윤활제의 오염 결정, 유압 유체(hydraulic fluids), 연료, 디젤 배출 내의 탄소 미립자 (carbon particulate) 그리고 엔진 블록, 트렌스미션(transmissions), 캠샤프트(camshafts) 와 크랭크샤프트 (crankshafts)내의 잔여물을 모니터링.

오염 물질의 특성에 대한 이러한 의무 규정들은 산업의 각 지점에 해당하는 표준에 상응하여 정의되어 있습니다.  허용할 수 있는 잔류물의 양은 매우 광범위하게 고려됩니다.

Features and Benefits:

• Fast, accurate and repeatable particle counting and classification
• Hands free operation requires no operator intervention
• Fully automated with integrated microscope, camera, stage and software
• Automated background correction
• Flexible detection for particles down to the micron level
• Complete archiving of all data
• Automated report generation
• International standards compliant

Particle Inspector

 

올림푸스 청정도 검사 시스템 “Particle Inspector”

청정도는 많은 산업, 제약 및 의료 장비 분야에서 매우 중요합니다.
청정도는 모든 제품 공정과 기술 구성요소의 기능 생명 주기에 영향을 미칠 수 있으며 모든 제품은 잔여물과 입자 검출에 대한 특정한 요구 사항을 가지고 있으므로 궁극적으로 제품 및 생산 공정의 품질 개선을 위해 사용될 수 있습니다.

올림푸스 “Particle Inspector” 는 완전히 자동화된 입자 분석, 분류 및 문서화에 대한 포괄적인 시스템입니다. 이 시스템은 현미경, 디지털 카메라 와 컨트롤러를 포함한 전동 스테이지 그리고 입자 분석 소프트웨어로 구성되어 있습니다. 입자의 임계값 기반의 평가는 입자의 영역, 크기, 형상, 위치, 밀도와 휘도와 같은 입자의 특정한 측정 항목을 제공하며 제한되어 선택된 영역 및 개체 분류로 평가할 수도 있습니다. 프레임 독립 검출은 복잡한 데이터 설정들을 최소화하면서 필드 밖의 입자들을 합쳐 주고 정확하게 정량화 합니다.

 

또한 올림푸스 자동화 필터 검사 시스템, 자동화된 입자 분석 시스템은 여러 가지 스캔 경로 및 예측 포커스 지도 정의 뿐만 아니라 추가적인 측정 항목들도 고려합니다. 현미경은 BX 정립형 현미경 (고 분해능 및 1마이크로미터보다 큰 작은 입자의 인식을 위해서) 과 SZX 연구용 실체 현미경 (큰 입자 및 빠른 검사를 위해)을 사용합니다.

Features and Benefits:

• Fast, accurate and repeatable particle counting and classification
• Hands free operation requires no operator intervention
• Fully automated with integrated microscope, camera, stage and software
• Automated background correction
• Flexible detection for particles down to the micron level
• Complete archiving of all data
• Automated report generation
• International standards compliant : VDA 19, ISO 16232-10, ISO 4406/4407, NF-E-48-651/655, STD 107-0002, US 788

Virtual Microscope “dotslide”

 

dotslide 멀티미디어 데모

dotslide 동영상 삽입

 

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dotslide 소개

Olympus Virtual Microscope; “.slide” 는

병리선생님들의 업무의 효율성과 권익을 보장할 수 있는 최상의 디지털 슬라이드 솔루션을 제공합니다.

이미지 삽입

VirtualMicroscope 란…

Virtual Microscope란, 진단용 유리슬라이드를 200, 400, 1000배의 고해상도 디지털 이미지로 스캐닝하여 컴퓨터를 통하여 진단 및 실습에 활용할 수 있도록 하는 가상디지털현미경으로, 인터넷이 연결된 곳이면 언제 어디서든 현미경 이미지를 확인 할 수 있는 장비입니다.

Digital Virtual Slide Image 생성과정

이미지 삽입

 

Virtual Microscope의 특장점

Virtual slide = High Quality

Virtual slide = All Magnification & High Resolution

Virtual slide = Always Focus

Virtual slide = Last Forever

Virtual slide = Anytime & Anywhere

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dotslide 네트워크 서버

 

Test dotslide online Database (opens a new window)
Find out for yourself how efficiently you can work with the ‘virtual slides’ generated using .slide. Check out the sample database online to gather your own impressions of the system under routine conditions.

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dotslide Specification

Specifications 및 시스템 구성

.slide – System structure

Resolution
Objective (IPLSAPO)	2	10	20	40
Numerical Aperture (N. A.)	0.08	0.16	0.75	0.90
Pixel resolution [µm]	3.22	0.64	0.32	0.16
PPmm (Pixels Per mm)	~300	~1,500	~3,100	~6,200
PPI (Pixels Per Inch)	~7,900	~39,000	~79,300	~158,700
비압축된 이미지 크기 (15 mm x 15 mm)	~66 MB	~1.6 GB	~6.5 GB	~26 GB
이미지 크기 (고품질 압축)	~5.5 MB	~140 MB	~540 MB	~2.2 GB
이미지 크기 (고 압축)	~2.7 MB	~65 MB	~260 MB	~1.1 GB

 

이미지 크기 및 스캐닝 속도

· 최대 스캐닝 속도 8 백만 화소/sec · 최대 전송 속도 24 MB/sec

 

Scan process (15mm x 15mm 시편 크기)

· 2x objective ~15 sec (overview scan) · 10x objective ~120 sec · 20x objective ~6:30 min · 40x objective ~25:00 min · Sample detection ~5 sec

 

Focus map

· The speed depends on the specimen and the number of focus points; approx. 45 sec

 

압축

· 데이터 압축은 어떠한 시간 지연 없이 스캐닝 프로세스 중에 진행됩니다.   고 품질및 고 압축을 선택할 수 있습니다.

 

포커싱

· 자동

 

Tissue finding

· 자동

 

Image Montage

· Seamless stitching

 

Hardware components

· .slide microscope, BX51 frame 대물렌즈: 2x, 10x, 20x, 40x UPLSAPO ( 추가 혹은 다른 렌즈로 교체 가능) 광원: brightfield with 100 W halogen lamp house 선택사항: polarization, fluorescence

· .slide motorized scanning stage   반복정밀도(Repeatability) < 1 µm Slide holder for 1” x 3” slides (default) optional: 2” x 3” slides 홀더 혹은 다른 치수 사용가능 PCI DSP stage controller card

· .slide CCD 카메라   2/3” CCD camera, 6.45 µm x 6.45 µm pixel size, high sensitivity, high resolution, Peltier cooled

· .slide 워크스테이션 (workstation)   with Dual XEON™ technology, Network interface 100/1000 Mbit/s Ethernet 4 GB RAM and high resolution 20.1” TFT monitor

· optional   slide loader for 50 3” x 1” slides, integrated barcode scanner

· optional   IT data storage solution

 

Specifications are subject to change without any obligation
on the part of the manufacturer.

Live Cell station “Cell”

 

OLYMPUS Cell^R

The Next Generation Live Cell Imaging

INSIGHTS INTO THE SECRETS OF LIFE

 

Advanced applications in live cell imaging

Microscopy in bioscience has progressed from the purely structural characterisation of fixed cells towards the investigation of processes in living cells with recent advances in fluorescence technology. Static morphological observation can now be complemented by the characterisation of the 3-D architecture of cellular structures and the real-time investigation of dynamic molecular processes in living cells. Newly developed fluorescence methods such as TIRF and FRET microscopy or GFP labelling are pushing the frontiers and widening the scope of bio-imaging.

Time-lapse Imaging

Dynamic processes such as cell growth, metabolic transport and signal transduction are monitored routinely nowadays. The duration of such processes may vary from the sub-second range to hours or even days. Consequently it may be necessary to take several images per second or just one image every couple of minutes.

Cell division in the early C. elegans embryo, microtubules in red, DNA in blue.
Courtesy of K. Oegema, T.Hyman group, Max-Planck Institut, Dresden, Germany.

Multi-colour and GFP Imaging

The development of a growing list of specific fluorochromes covering the entire colour range enables the scientist to image and distinguish different sub-cellular structures simultaneously within one experiment through the use of multiple staining. If this is combined with time-lapse acquisition, the illumination unit of the microscope must be able to switch quickly between excitation wavelengths

Z-sectioning and Multi-dimensional Imaging

Microscopy is basically a two-dimensional observation technique while biological samples are three-dimensional. Therefore, in order to map the entire volume of the specimen, it can be imaged in layers by moving the focal plane in precise steps using a motorised Z-drive or a piezo-electric objective drive.

Ion Imaging / Ratio Imaging / Ca++ Imaging

The fluorescence behaviour of several dyes is dependent on the concentration of certain ions such as calcium (Fura-2) or on the pH value (BCECF). The detection,
quantification and analysis of changes in fluorescence intensity are thus an indirect means to study important physiological processes.

Time-lapse imaging:Fura2-labelled HeLa cells stimulated with APT.
Top : dual-excitation image; below; false-color ratio images revealing increasing calcium concentration.

FRET (Foerster Resonance Energy Transfer)

The measurement of fluorescence energy transfer from a fluorochrome molecule to an adjacent one can be used for the investigation of molecular interactions in cells. It requires the acquisition of images with different excitation and emission wavelengths and sophisticated correction algorithms.

TIRFM (Total Internal Reflection Fluorescence Microscopy)

Investigating surfaces without interference from background light can be carried out using Total Internal Reflection Fluorescence Microscopy. Laser light coupled together with the standard fluorescence excitation allows fast switching between TIRF and wide-field fluorescence applications and can even support simultaneous observation.

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cell^tool TIRFM System

cell^tool TIRFM is based on a modular multi-port illuminator for up to three lasers and a MT10 or MT20 widefield fluorescence light source. This extension of the cell^M and cell^R imaging stations allows for laser based high resolution cell surface and membrane studies with the possibility of simultaneous widefield observation. The control of the TIRFM illuminator is integrated into the ‘Experiment Manager’ of cell^M and cell^R software, so in addition to highest quality TIRF observation cell^tool TIRFM offers all the powerful options of the cell* imaging stations.
The cell^tool TIRFM is available as a complete turn-key solution or as an add-on for existing cell^M or cell^R imaging systems.

Cell surface observation without out-of-focus blur
Fully integrated into cell^M and ^R imaging stations
Combination of up to three lasers plus a MT10/20 fluorescence illumination system
Optimised beam alignment on individual laser ports

Single molecule fluorescence detection with TIRFM

Commencing as a challenging problem in physics with the first detection of a single fluorescent molecule in condensed phase at temperatures of liquid helium single molecule fluorescence detection has diversified into a collection of methods applied in various scientific disciplines.
With the advent of ultra sensitive detectors and optical instrumentation and by combination with confocal and TIRFM techniques single molecule detection developed into a feasible approach in life science. Decisive for this adaptability is the possibility to detect single molecule fluorescence at room temperature in solution (e.g. FCS) or on surface membranes of even living cells (TIRFM).

The following experiment conducted on an inverse microscope using the Olympus UAPO150xO/TIRFM objective is an example of the many applications for TIRFM single molecule fluorescence detection:
Single stranded RNA hybridised to a complementary biotinylated DNA, which was immobilised on a BSA-Biotin-Streptavidin coated cover glass. The RNA was mono-labelled with Cy3. Imaging of single molecules was confirmed by single step photo bleaching of the dye. Emission intensity plotted versus time decays immediately after bleaching a single dye molecule (ROIs 2-5), contrary to a group of fluorescent molecules whose emission would decrease in a multistep exponential manner (ROI 1).

Figure 1: Fluorescence intensity (colour coded. Circles mark five regions of interest (ROI). Each ROI (except of ROI 1) contains one single fluorescent molecule as verified by single step photobleaching: See movie with fluorescence intensity recorded over time (to download the film click on figure 1) and corresponding emission intensity curves plotted vs. time for the selected ROIs (Figure 2, bottom of the page).

Total internal reflection (=TIR)

TIR is an optical phenomenon. If light is travelling through a medium with a high refractive index and strikes the interface of an optical medium with a lower refractive index at an angle greater than the critical angle, the incident light will undergo total internal reflection. Under these conditions some light still enters the low refractive index medium as an electromagnetic wave termed the evanescent wave. The intensity of this wave decays exponentially with penetration depth. The average z-expansion is less than 200 nm depending on the wavelength of light, the incident angle and the refractive index of the media. In TIRFM, fluorophores in the sample at a maximum distance of ~100 nm from the coverslip surface are selectively excited. This z resolution of 100 nm is one-fifth of an optical section obtained with a laser scanning confocal microscope. Therefore TIRFM is ideally suited for the observation of processes and structures on or close to the cell surface.

Total Internal Reflection Fluorescence Microscopy

Total internal reflection (=TIR) is an optical phenomenon. If light is travelling through a medium with a high refractive index and strikes the interface of an optical medium with a lower refractive index at an angle greater than the critical angle, the incident light will undergo total internal reflection. In biological investigations involving living specimen, these prerequisites are met if a laser beam travels through glass, for example a microscope slide (refractive index n = 1.52), towards an aqueous buffer solution or cell surface (n = 1.33 – 1.38). The beam is totally reflected at the interface between the glass and the medium or the cell surface respectively. But the reflected light generates an electromagnetic wave termed the evanescent wave which enters the low refractive index medium. The intensity of this wave decays exponentially with penetration depth along the z-axis. The penetration depth depends on the wavelength of light, the incident angle and the refractive index of the media. The thickness of the optical section that generates fluorescence under TIR can be adjusted by changing the incident angle and has a range between a few hundred and 50 nm at minimum. That is up to one-tenth of an optical section obtained with a confocal laser scanning microscope. Thus in TIRFM, fluorophores close to the coverslip surface can be selectively excited. As in confocal microscopy background fluorescence is nearly absent in TIRFM, because the fluorophore excitation is restricted to the focal plane of the objective. Therefore TIRFM gives high-contrast images of the cell surface with excellent signal-to-background ratio and is ideally suited for the observation of processes and structures on the cell surface and within or close to the plasma membrane.
In contrast, widefield fluorescence microscopy provides limited spatial resolution in z-direction because background fluorescence from outside the focal plane often impedes the detection of small or weakly fluorescent structures.
TIRFM does not require the rather expensive scanning microscope technique because it generates widefield illumination at the specimen surface. It can be performed relatively cost-efficient with inverted fluorescence microscopes equipped with a special episcopic fluorescence illuminator, special high NA objectives and a laser.
Confocal microscopy has the clear advantage in not being restricted to the optical section directly adjacent to the coverslip/specimen interface. However optical sections obtained by TIRFM can be up to one order of magnitude thinner than by confocal microscopy. Further-more, being a widefield technique, TIRFM comprises the considerable advantages of a higher acquisition speed as compared to scanning microscopy.

The TIRF Microscope

TIRFM is a widefield microscopy technique and consequently modified fluorescence microscope set-ups are used. Lasers are commonly employed as illumination sources because the light is coherent, polarised, intense and well collimated. If a special dual port epi-fluorescence condenser is used the change from widefield illumination with a standard arc lamp to the TIRF laser is rapid and straightforward and does not interfere with the beam alignment.
For TIRFM with inverted microscopes, the incident beam is focused off-axis at the objective back focal plane such that it passes the very periphery of the pupil of a highly refractive objective. The objective’s numerical aperture must be at least 1.38 to exceed the refractive index of a living cell. Thus the incident beam emerges from the front lens into the immersion oil in such a way that it reaches the glass/cell interface in a critical angle and undergoes total internal reflection. The off-axis position of the laser beam determines the incident angle and the depth of the evanescent field accordingly. The further off-centre the alignment is, the larger is the angle and the shallower is the evanescent wave.
Olympus offers a range of special TIRF objectives with very high numerical apertures (NA). There are three objectives available with a NA of 1.45 that can be used with conventional immersion oil and cover slips. With the PLAPON60xO/TIRFM-SP, the PLAPO100XO/TIRFM-SP or the new UAPO150xO/TIRFM-SP a penetration depth of the exciting wave around 100 nm is reached. The UAPO150xO/TIRFM is especially designed for single-molecule detection in TIRFM. The APO100XOHR features an unsurpassed NA of 1.65. To match the extreme high NA special high-refraction cover slips and special immersion liquid (diiodomethane) are required. Therefore the objective offers a unique short penetration depth of about 50 nm and high flexibility regarding the marginal angle available for TIR. The 60x and the 150x objec-tives have a temperature correction collar. Common plan apochromatic objectives like 100x with NA = 1.40 can also be employed for TIRFM, but they are less convenient due to the small angle that is available for the total internal reflection.

 

Applications

The visualisation of molecular interactions on surfaces is of fundamental interest in cell and molecular biology because many molecular transport and signal transduction processes are transmembrane incidents. Examples are binding and triggering of cells by hormones, neurotransmitters and antigens, cell adhesion to surfaces, electron transport in the membrane, cytoskeletal and membrane dynamics, cellular secretion events and vesicular fusion events with membranes. TIRFM is the perfect tool for visualisation of these processes. The extraordinary small depth of field of TIRFM assures that only surface-bound fluorophores are detected. Fluorophores in the surrounding medium remain invisible though they might be in rapid exchange and be present in large excess. This is different from normal widefield illumination where they would create overpowering background fluorescence.
During many experiments it might be desirable to switch rapidly between TIR illumination to observe the surface and standard epi-illumination to investigate the deeper layers of the specimen as well. For example, a transient process may involve simultaneous but not identi-cal processes in the membrane and the cytoplasm. The Olympus BioSystems dualport illuminator for combined TIR and widefield illumination allows the fast and synchronised alternation of the two techniques. The techniques of TIRFM, DIC and other microscopy techniques as FRET, FRAP and FLIM can also be combined.

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Cell Software 소개

준비중입니다.

 

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Cell Hardware 소개

Cell Hardware features

1. MT20 광원 장치

Cell 시스템에서 가장 중요한 장치 중 하나인 광원 장치는 MT20 광원 시스템을 사용합니다.

 MT20 광원 장치는 두 가지 타입의 램프 (Xeneon 혹은 Xe/Hg)을 사용할 수 있으며 전원 장치가 내장되어 뛰어난 광량의 안정화를 이루었습니다.

또한 내장된 8개의 필터 장착 휠은 고속의 회전으로 즉각적인 필터 변경을 가능케 하며 14단계의 광량 감쇄기 휠 뿐만 아니라 1ms의 고속 셔터도 지원합니다.

• MT20 만의 유일한 기능

1. 병렬 처리
– 필터 교체
– 셔터 개폐
– 광량 조절
시편의 Bleaching 감소를 가능하게 합니다.

2. 광원 소스의 안정성 증대
정량 분석에 필수적인 조건

3. 사용자 친화적
도구가 필요하지 않은 필터 장착

[ MT20 광원 시스템의 광학 설계 ]

2. 실시간 제어기 (Real-Time Controller)

Cell 시스템에서 또 하나의 매우 중요한 장치로 이미징 워크스테이션 내의 실시간 제어기 (real time controller)보드가 있습니다.

이 실시간 제어기가 광원 장치, 카메라 및 현미경의 제어를 실시간으로 하드웨어적인 처리를 제어 함으로써 시편의 Bleaching을 극소화 할 수 있습니다.

또한 Time point 기반의 실험에서 1us 단위의 매우 정확한 Time point를 보증할 수 있게 하여 분석 결과의 신뢰성을 높여 줍니다.

[ 실시간 제어기의 이점 ]

A. 필터 휠 (Filter wheel)을 사용한 일반적인 이미징 시스템

하나의 이미지 쌍을 획득하는데 650ms 이상의 시간이 걸림

B. Cell^R 병렬 처리 시스템

Cell^R 실시간 처리기 사용시 최소 2배 이상 빠름

위의 처리 순서 비교 이미지를 보면 셔터 개폐 시간 10ms, 카메라 노출 시간 50ms, 카메라 Readout (데이터 전송) 시간 80ms, 필터 교체 시간 50ms 기준으로 두 시스템간의 차이를 비교 한 것입니다.

일반적인 순차 처리 방식의 시스템의 경우 PC에서의 지연 시간 및 필터 교체등에 의한 진동의 영향을 받게 됩니다.

Cell 시스템은 실시간 처리기를 사용함으로써 일반적인 순차적인 제어가 아닌 병렬적인 동시 제어가Hardware적으로 가능함을 알 수 있습니다.

이는 더 장시간의 더 많은 이미지를 Bleaching이나 Cell Dead가 없이 정량적 분석이 가능함을 나타내며 동일 조건시에 더 많은 노출 시간을 확보할 수 있음으로 카메라의 영상 품질의 향상 역시 기대할 수 있습니다.

위 이미지에서 샘플에 형광 Excitation이 노출 되는 시간을 비교하면 A의 경우 170msec, B의 경우 50msec 로 1/3 이하의 노출 만으로 동일한 조건의 이미지를 획득할 수 있게 됩니다.

Solution Systems

 

※ Solution Systems 제품입니다. 클릭하시면 자세한 내용을 확인하실 수 있습니다.

– Virtual Microscope “dotslide”

– Live Cell station “Cell”

– 청정도 검사 시스템

– 광학 필터 분석 시스템

– 비금속 개재물 분석 시스템

– cell^TIRF