J.H.Jin의 모든 글

Chroma, Filter, Fluorescence

49021

 JNOPTIC Filter 49021

Download Catalog_49021

49021 Spectrum

Filters Type
ET405/20x EX
 T425lpxr BS
ET460/50m EM

 

– Filter set for BFP

  • Main Fluorochromes : EBFP
Excitation (㎚) Emission (㎚)
385 448

 

Spectrum Comparison Fluor and 49021

BFP VS 49021

 

Best with these fluorochromes

 

49021 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

Chroma, Filter, Fluorescence

49009

 JNOPTIC Filter 49009

Download Catalog_49009

49009 Spectrum

Filters Type
ET640/30x EX
 T660lpxr BS
ET690/50m EM

 

– Filter set for Cy5 Narrow Excitation

The narrow-band excitation provided by this filter set is useful in applications where fluorochromes such as Texas Red or AlexaFluor 594 are also present in the same sample. This set will minimize bleedthrough of these fluorochromes. Best FISH filter set for Far Red probes.

  • Main Fluorochromes :Cy5
Excitation (㎚) Emission (㎚)
649 670

 

Spectrum Comparison Fluor and 49009

Cy5 VS 49009

 

Best with these fluorochromes

 

49009 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Filter, Fluorescence

49008

JNOPTIC Filter 49008

Download Catalog_49008

49008 Spectrum

Filters Type
ET560/40x EX
 T585lpxr BS
ET630/75m EM

 

– Filter set for Alexa Fluor 594, mCherry, Texas Red

Excitation (㎚) Emission (㎚)
590 617

Spectrum Comparison Fluor and 49008

Alexa Fluor 594 VS 49008

 

Best with these fluorochromes

 

49008 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

Chroma, Filter, Fluorescence

49007

JNOPTIC Filter 49007

Download Catalog_49007

49007 Spectrum

Filters Type
ET710/75x EX
 T760lpxr BS
ET810/90m EM

 

– Filter set for Cy7

  • Main Fluorochromes : Cy7™
Excitation (㎚) Emission (㎚)
743 767

 

Spectrum Comparison Fluor and 49007

Cy7 VS 49007

 

Best with these fluorochromes

 

49007 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Filter, Fluorescence

49002

JNOPTIC Filter 49002

Download Catalog_49002

49002 Spectrum

Filters Type
ET470/40x EX
 T495lpxr BS
ET525/50m EM

 

– Filter set for EGFP (FITC/Cy2)

  • Main Fluorochromes : GFP
Excitation (㎚) Emission (㎚)
488 507

 

Spectrum Comparison Fluor and 49002

GFP VS 49002

 

Best with these fluorochromes

 

49002 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

 

Chroma, Filter, Fluorescence

39009

JNOPTIC Filter 39009

Download Catalog_39009

39009 Spectrum

Filters Type
AT480/30x EX
 AT505DC BS
AT620/60m EM

 

– Filter set for Acridine Orange/Di-8-ANEPPS

  • Main Fluorochromes : EtBr
Excitation (㎚) Emission (㎚)
520 603

 

Spectrum Comparison Fluor and 39009

EtBr VS 39009

Best with these fluorochromes

  • Acridine Orange + RNA
  • Di-8-ANEPPS non-ratiometric
  • DiA
  • Ethidium Bromide
  • FM™ 1-43
  • LysoTracker Yellow HCK-123
  • Pulsar™ 650

 

39009 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes
Chroma, Filter, Fluorescence

39003

JNOPTIC Filter 39003

Download Catalog_39003

39003 Spectrum

Filters Type
AT495/20x EX
 AT515DC BS
AT540/30m EM

 

– Filter set for EYFP/Venus/Citrine

  • Main Fluorochromes : YFP
Excitation (㎚) Emission (㎚)
514 527

 

Spectrum Comparison Fluor and 39003

YFP VS 39003

 

Best with these fluorochromes

 

39003 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

Chroma, Filter, Fluorescence

39001

JNOPTIC Filter 39001

Download Catalog_39001

39001 Spectrum

Filters Type
AT435/20x EX
 AT455DC BS
AT480/30m EM

 

– Filter set for ECFP/Cerulean

  • Main Fluorochromes : CFP
Excitation (㎚) Emission (㎚)
433 475

 

Spectrum Comparison Fluor and 39001

CFP VS 39001

 

Best with these fluorochromes

  • AmCyan
  • Atto 425
  • Cerulean
  • CFP
  • ECFP

 

39001 Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes

 

Fluorescence, Fluorochrome

Cy5.5

Spectrum Chart

Cy5.5 Spectrum
Excitation (㎚) Emission (㎚)
675 695

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49022 545/25
(532.5~557.5)		 565 (LP) 605/70
(570~640)		 Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49914 642/20
(632~652)		 647 (LP) 705/72
(669~741)		 For use with laser between 633~650㎚

Narrow Excitation band,
Bandpass Emission

Information

A variety of cyanine 5.5 (Cy5.5) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5 dyes are one type of the most common red fluorophores.

Cyanine 5.5 (Cy5.5) 염료는 형광 이미징 및 기타 형광 기반 생화학 분석을 위해 생물 분자를 표지하는 데 사용되었습니다. 그들은 펩타이드, 단백질 및 올리고 제 등의 라벨링에 널리 사용됩니다. Cy5.5 염료는 가장 일반적인 적색 형광체의 한 유형입니다.

Fluorescence, Fluorochrome

tdTomato

Spectrum Chart

Excitation (㎚) Emission (㎚)
554 581

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)		 565 (LP) 605/55
(577.5~632.5)		 Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)		 565 (LP) 605/70
570~640)		 Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)		 565 (LP) 590/33
(573.5~606.5)		 Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49908 532/10
(527~537)		 532 (LP) 590/50
(565~615)		 For use with laser between 529~535㎚

Narrow Excitation band,
Longpass Emission
49909 561/10
(556~566)		 561 (LP) 600/50
(575~625)		 For use with laser between 557~563㎚

Narrow Excitation band,
Bandpass Emission

Information

tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation. Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato. Its excitation and emission maxima occur at 554 ㎚ and 581 ㎚, respectively. Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.