mCherry is a fluorophore (a fluorescent protein) used as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from the protein drFP583 (commonly known as DsRed) isolated from Discosoma sea anemones, while other fluorescent proteins in the green range are often variants of jellyfish GFP. DsRed is a tetramer optimally excited at 558 ㎚ and has an emission maximum at 583 ㎚.
Our patented slicer technology allows you to double your slice viability for healthier tissue. The Compresstome® produces beautiful sections while providing a fast user-friendly cutting experience. Please refer to our online video tutorials for a step by step visual aid in your Compresstome® experience.
The Compresstome® VF-200 is our best semi-automatic tissue slicer used to section live tissue for electrophysiology and imaging studies. If you are looking to section both fresh and fixed tissue for immunohistochemistry, please explore the VF-300-0Z.
The VF-200 is our semi-automatic tissue slicer most ideal for:
The Compresstome® VF-200-0Z is our entry level Compresstome®. With our patented compression technology, this semi-automated slicer excels at sectioning slices used for:
Our patented slicer technology allows you to double your slice viability. The Compresstome® is also easy to use, and our online training videos will allow everyone in your lab to produce excellent slices.
The Compresstome VF-200-0Z is best used to section live tissue for electrophysiology and imaging studies. If you are looking to section fixed tissue for immunohistochemistry, please explore the VF-300-0Z.
With our patented compression technology, this semi-automated slicer excels at sectioning slices used for:
The VF-300 is the workhorse of the Compresstome®family. With our compression technology, this fully automated slicer excels at sectioning slices used for:
Our slicer technology allows you to double your slice viability. The Compresstome® is also easy to use. Our online training videos will allow everyone in your lab to produce excellent slices.
At Precisionary Instruments, we aim to push the boundaries of scientific research. That’s why we love to see how scientists find new ways to use our products.
At the Allen Institute, the “open science” concept pairs neuroscientists with system engineers to come up with innovative ideas and conjure them into existence. We are proud that the Compresstome® can be part of this project, helping the scientists work with both mouse brains as well as human brain tissues.
Products we recommend to purchase with VF-300-0Z:
To reduce contamination when working with both fresh and fixed tissue we recommend purchasing additional:
The VF-300-0Z Compresstome® from Precisionary Instruments represents the latest advances in vibratome technology. Our patented compression technology enables our vibratome to overpower the competition. This gentle, uniform compression from the agarose embedding process helps to stabilize the tissue during sectioning and allows our vibratome to produce smoother and healthier slices than the competition. The VF-300-0Z is ideal for electrophysiology, immunohistochemistry, Precision-cut tissue slices, and organotypic cultures.
The main advantages of the Compresstome® include:
Patented compression technology and agarose embedding process stabilizes tissue and eliminates chattermarks
Slices over 5x faster than other market Vibratomes
Preserves surface neurons yielding over 2x healthy/dead cell ratios and significantly improving patch clamping results for electrophysiology
Slice thickness ranging from 4 μm-2000μm
No freezing needed! Allowing for free-floating staining for better IHC – lmmunohistochemistry results
Fully automated slicing, so no more tedious cryostat cranking
Auto Zero-Z® technology reduces z-axis deflection to <1 μm
Light footprint for increased portability
The VF-300-0Z is the Auto Zero-Z® version of our workhorse Compresstome®. With our compression technology, this fully automated slicer excels at sectioning slices used for:
Our slicer technology allows you to double your slice viability. The Compresstome® is also easy to use. Our online training videos will allow everyone in your lab to produce excellent slices.
At Precisionary Instruments, we aim to push the boundaries of scientific research. That’s why we love to see how scientists find new ways to use our products.
At the Allen Institute, the “open science” concept pairs neuroscientists with system engineers to come up with innovative ideas and conjure them into existence. We are proud that the Compresstome® can be part of this project, helping the scientists work with both mouse brains as well as human brain tissues.
Products we recommend to purchase with VF-300-0Z:
To reduce contamination when working with both fresh and fixed tissue we recommend purchasing additional:
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-U(L), B(L), G(L) Camera : AcquCAM 23GR2 C-mount adapter : 1X Lens : UPLNAPO40X Above three images are merged and processed using ARM S/W
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-U(B), B(B), G(B) Camera : AcquCAM 23GR2 C-mount adapter : 1X Lens : PlanApo60xWLSM Above three images are merged and processed using ARM S/W
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-U(L), B(L), G(L) Camera : AcquCAM 23S2 C-mount adapter : 1X Lens : UPLNAPO40X Above three images are merged and processed using ARM S/W
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-U(B), B(B), G(B) Camera : AcquCAM 23S2 C-mount adapter : 1X Lens : PlanApo60xWLSM Above three images are merged and processed using ARM S/W
CA3 pyramidal neuron, IR-DIC & Alexa Fluo 488 at the same time Figure courtesy by Dr. Sooyun Kim, Seoul National University with Bx51WI, JNO-DPTS, AcquCAM 23S
JNO-DPTS CA3 pyramidal neuron, IR-DIC & Alexa Fluo 488 at the same time Figure courtesy by Dr. Sooyun Kim, Seoul National University with Bx51WI, JNO-DPTS, AcquCAM 23S
USB 3.0 Monochrome Camera
1/1.2 ” Pregius Sensor
1920×1200 pixel
Trigger input and I/O
Cost-effective mono camera for fluorescence microscope (Best)
Cost-effective camera for Department of Physiology (Best)
1/1.2 inch optimized sensor size for microscope field of view
Acquire images of optimum width combined with 0.63x adapter
AcquCAM 23S2 mono camera has a USB 3.0 interface and is the perfect solution for many industrial automation, quality assurance, security, surveillance and medical applications. The mono camera ships with the very sensitive 1/1.2 inch CMOS Pregius sensor. With up to 162 images per second, the AcuqCAM 23S2 is a low cost, yet highly versatile imaging solution. The camera includes a C to CS mount adapter, making it compatible to C and CS mount lenses.
Images with AcquCAM 23S2
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-U(B), B(B), G(B) Camera : AcquCAM 23S2 C-mount adapter : 1x Lens : UPlanAPO 40x Above three images are merged and processed using ARM S/W
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-U(B), B(B), G(B) Camera : AcquCAM 23S2 C-mount adapter : 1x Lens : UPlanAPO 40x Above three images are merged and processed using ARM S/W
Specification
Spec
Description
Size
1/1.2 inch
Resolution
H: 1920, V: 1200
Pixel size
H: 5.86 µm, V: 5.86 µm
Video formats & Frame rate
1920×1200 Y16 @ 80 fps 1920×1200 Y800 @ 162 fps
Sensitivity
0.015 lx
Exposure time
20㎲ to 30 s
Lens mount
C/Cs mount
Interface
USB 3.0
Power supply
4.5 to 5.5 VDC
Dimensions
H: 29 mm, W: 29 mm, L: 43 mm
Mass
65 g
ARM
ARM is image analysis software for JNOPTIC AcquCAM cameras. This S/W is interchangeable with all WDM cameras regardless of camera brand and model and even more the most strength point is simple and easy use of length, area, angle, etc.
