Fluorescence In Situ Hybridization
– 형광동소보합법 –
Inadequat Contrast Image Good Contrast Image Inadequat Contrast Spectrum Chart Good Contrast Spectrum Chart
Many factors influence the quality of FISH
1. Processing & storage | 2. Specimen | 3. Probes |
4. Microscope | 5. Filters | 6. Light source |


필터 선정시 고려 할 사항 | 설 명 |
---|---|
Contrast | Signal / Noise ratio = MORE CONTRAST |
Brightness | Save time, increase throughput and reduce eye strain with brighter images |
Color Separation | Distinguish between colors more easily and exclude unwanted colors |
Registration | Each filter set produces images that are aligned with images from other filter sets |